5J5E
crystal structure of antigen-ERAP1 domain complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-11 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.260, 66.850, 66.500 |
| Unit cell angles | 90.00, 110.16, 90.00 |
Refinement procedure
| Resolution | 62.430 - 2.800 |
| R-factor | 0.20987 |
| Rwork | 0.207 |
| R-free | 0.26890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rjo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.610 |
| Data reduction software | iMOSFLM (7.2.0) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.430 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.335 | |
| Number of reflections | 10114 | |
| <I/σ(I)> | 6.6 | 2.3 |
| Completeness [%] | 77.0 | 81.3 |
| Redundancy | 2.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 100 mM Tris, pH 8.5 and 16% PEG8000 |
