5J57
V5E1-RTA complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.692, 68.216, 71.570 |
| Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
| Resolution | 33.631 - 1.700 |
| R-factor | 0.1644 |
| Rwork | 0.163 |
| R-free | 0.18970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.816 |
| Data reduction software | HKL-2000 (v1.0) |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.7.1_743) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 33.631 | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 4.610 | 1.700 |
| Rmerge | 0.061 | 0.042 | 0.371 |
| Total number of observations | 127807 | ||
| Number of reflections | 39982 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 97.6 | 92.6 | 93.5 |
| Redundancy | 3.2 | 3.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 100 mM Tris pH 7.0 and 20% PEG 1000 |
