5J56

RTA-V1C7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 32 2 1
Unit cell lengths	64.768, 64.768, 215.016
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.729 - 1.800
R-factor	0.1752
Rwork	0.173
R-free	0.20960
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	1.609
Data reduction software	HKL-2000 (v1.0)
Data scaling software	SCALEPACK
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.729	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.060	0.040	0.996
Rmeas	0.064	0.043
Rpim	0.022	0.015	0.370
Total number of observations	393312
Number of reflections	49957
<I/σ(I)>	5.5
Completeness [%]	99.9	99.9	99.9
Redundancy	7.9	8.3	7.6
CC(1/2)		0.997	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293	100 mM sodium acetate pH 4.6 and 8% PEG4000