5J4V

The crystal structure of Inhibitor Bound to JCV Helicase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	105
Detector technology	CCD
Collection date	2013-03-27
Detector	ADSC QUANTUM 315
Wavelength(s)	1.12709
Spacegroup name	P 6
Unit cell lengths	109.920, 109.920, 66.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.960 - 2.940
R-factor	0.169
Rwork	0.165
R-free	0.24700
RMSD bond length	0.010
RMSD bond angle	1.090
Data scaling software	Aimless (0.1.27)
Refinement software	BUSTER (2.11.6)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	54.960	54.960	2.946
High resolution limit [Å]	2.936	13.560	2.936
Rmerge	0.096	0.022	0.366
Number of reflections	9135
<I/σ(I)>	17.9
Completeness [%]	91.5	89.6	100
Redundancy	5.8	4.6	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	25-30% Peg400, 0.1 M LiSO4, 0.1M HEPES (pH 7.0), 0.2 M NaCl