5J4U
Crystal structure of a glutathione S-transferase PtGSTU30 from Populus trichocarpa in complex with GSH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.028, 74.646, 48.935 |
| Unit cell angles | 90.00, 121.87, 90.00 |
Refinement procedure
| Resolution | 40.850 - 1.249 |
| R-factor | 0.1701 |
| Rwork | 0.170 |
| R-free | 0.18110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4top |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.034 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.850 | 1.270 |
| High resolution limit [Å] | 1.249 | 1.250 |
| Number of reflections | 68741 | |
| <I/σ(I)> | 45.91 | |
| Completeness [%] | 98.5 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291.15 | 1.8M Ammonium sulfate, 0.1M Sodium citrate tribasic dehydrate, pH 5.2 |
