5J4I
Crystal Structure of the L-arginine/agmatine antiporter from E. coli at 2.2 Angstroem resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.739, 175.424, 73.148 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.505 - 2.207 |
| R-factor | 0.1978 |
| Rwork | 0.197 |
| R-free | 0.21010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ob6 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | XDS (VERSION November 11, 2013) |
| Data scaling software | XSCALE (VERSION November 11, 2013) |
| Phasing software | MOLREP (Phaser) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.330 |
| High resolution limit [Å] | 2.207 | 2.210 |
| Rmerge | 0.071 | 1.140 |
| Number of reflections | 68197 | |
| <I/σ(I)> | 14.9 | 1.5 |
| Completeness [%] | 99.4 | 96.8 |
| Redundancy | 5.9 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 298 | PEG 400, NaCl |
