5J4I

Crystal Structure of the L-arginine/agmatine antiporter from E. coli at 2.2 Angstroem resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-03-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	104.739, 175.424, 73.148
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.505 - 2.207
R-factor	0.1978
Rwork	0.197
R-free	0.21010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ob6
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	XDS (VERSION November 11, 2013)
Data scaling software	XSCALE (VERSION November 11, 2013)
Phasing software	MOLREP (Phaser)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.510	2.330
High resolution limit [Å]	2.207	2.210
Rmerge	0.071	1.140
Number of reflections	68197
<I/σ(I)>	14.9	1.5
Completeness [%]	99.4	96.8
Redundancy	5.9	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	PEG 400, NaCl