5J4F
Crystal structure of the N-terminally His6-tagged HP0902, an uncharacterized protein from Helicobacter pylori 26695
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-12-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 33.512, 78.643, 41.404 |
| Unit cell angles | 90.00, 112.22, 90.00 |
Refinement procedure
| Resolution | 19.850 - 1.400 |
| R-factor | 0.18211 |
| Rwork | 0.181 |
| R-free | 0.20779 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2q30 |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 71.000 |
| High resolution limit [Å] | 1.400 |
| Rmerge | 0.054 |
| Number of reflections | 38998 |
| <I/σ(I)> | 15.8 |
| Completeness [%] | 99.8 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 3350, sodium chloride, Tris-HCl |
