5J4A

CdiA-CT toxin from Burkholderia pseudomallei E479 in complex with cognate CdiI immunity protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	70
Detector technology	CCD
Collection date	2013-06-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97591
Spacegroup name	P 2 21 21
Unit cell lengths	54.472, 73.260, 110.028
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.713 - 2.004
R-factor	0.196
Rwork	0.193
R-free	0.23660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.094
Phasing software	PHENIX (Phaser-MR: 1.10.1-2155)
Refinement software	PHENIX (phenix.refine (1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.000
Number of reflections	30121
<I/σ(I)>	12.1
Completeness [%]	99.2
Redundancy	10.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.98	293	0.1 M HEPES (pH 6.98), 0.02 M magnesium chloride, 30% (w/v) polyacrylic acid