5J43
CdiA-CT from uropathogenic Escherichia coli in complex with CysK
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 70 |
Detector technology | CCD |
Collection date | 2012-06-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 41 |
Unit cell lengths | 64.010, 64.010, 365.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.919 - 2.700 |
R-factor | 0.2016 |
Rwork | 0.200 |
R-free | 0.22420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oas |
RMSD bond length | 0.009 |
RMSD bond angle | 1.225 |
Data reduction software | iMOSFLM ((iMosflm: 7.2.1)) |
Phasing software | PHENIX ((Phaser-MR: 1.10.1_2155)) |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 64.010 |
High resolution limit [Å] | 2.700 |
Number of reflections | 39863 |
<I/σ(I)> | 9.9 |
Completeness [%] | 99.4 |
Redundancy | 12.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M sodium sulfate, 0.1 M bis-tris propane, pH 7.9, 20% PEG 3350 |