5J3Y
Crystal structure of S. pombe Dcp2:Dcp1 mRNA decapping complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-13 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 124.362, 124.362, 366.978 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.444 - 3.288 |
| R-factor | 0.2258 |
| Rwork | 0.224 |
| R-free | 0.25180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qkm |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.399 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.400 | 3.470 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.859 | |
| Number of reflections | 26491 | |
| <I/σ(I)> | 10.3 | 2.3 |
| Completeness [%] | 99.6 | 99.6 |
| Redundancy | 9.6 | 9.5 |
| CC(1/2) | 0.995 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 292 | 0.1 M MES/NaOH (pH 6.5), 0.2 M ammonium sulfate, 30% (w/v) PEG 5000 MME |
