5J3E
Crystal Structure of Human THYN1 protein in complex with 5-methylcytosine containing DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.327, 73.232, 108.201 |
| Unit cell angles | 90.00, 91.18, 90.00 |
Refinement procedure
| Resolution | 43.510 - 2.600 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eop |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.379 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.9) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.510 | 43.510 | 2.720 |
| High resolution limit [Å] | 2.600 | 9.010 | 2.600 |
| Rmerge | 0.094 | 0.024 | 0.729 |
| Rmeas | 0.110 | 0.028 | 0.850 |
| Rpim | 0.056 | 0.015 | 0.435 |
| Total number of observations | 73091 | 1674 | 9148 |
| Number of reflections | 19516 | ||
| <I/σ(I)> | 12.8 | 38.5 | 2.1 |
| Completeness [%] | 99.8 | 97.8 | 100 |
| Redundancy | 3.7 | 3.4 | 3.8 |
| CC(1/2) | 0.997 | 0.999 | 0.764 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M TRISODIUM CITRATE, 0.2M NaCl, 30% PEG3350 |
