5J3C

Thermus thermophilus 70S termination complex containing E. coli RF1

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-12-04
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97910
Spacegroup name	P 21 21 21
Unit cell lengths	209.510, 450.890, 622.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.864 - 3.040
R-factor	0.2479
Rwork	0.246
R-free	0.27850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4o
RMSD bond length	0.005
RMSD bond angle	0.895
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	49.870		3.120
High resolution limit [Å]	3.040	13.600	3.040
Rmerge	0.346	0.083	1.985
Number of reflections	1109069
<I/σ(I)>	6.46	19.48	1.59
Completeness [%]	99.3	96.9	98.5
Redundancy	12.7
CC(1/2)	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
2	VAPOR DIFFUSION, SITTING DROP	7	293	4.0% PEG 20k, 8.0% PEG 200, 200 mM TRIS-ACETATE, 200 mM KSCN, 10 mM MAGNESIUM ACETATE 2.8mM DOBC