5J2Z
PRV UL37 N-terminal half (R2 mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 51.281, 68.696, 156.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.723 - 2.500 |
| R-factor | 0.1834 |
| Rwork | 0.178 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k70 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.779 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.723 | 2.540 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.126 | 0.262 |
| Number of reflections | 19342 | |
| <I/σ(I)> | 14.8 | 3.5 |
| Completeness [%] | 96.5 | 67.4 |
| Redundancy | 5.6 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | 30% PEG 1000, 0.3M calcium acetate, 0.1M Imidazole, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
