5J2O

Crystal structure of the cyan fluorescence protein Cerulean S175G mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 21 21 21
Unit cell lengths	51.137, 62.898, 70.106
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.053 - 1.500
Rwork	0.169
R-free	0.18780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ydz
Data scaling software	HKL-2000 (1.97.2)
Phasing software	PHENIX (1.10.1-2155)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.053	1.530
High resolution limit [Å]	1.500	1.500
Rmerge		0.249
Number of reflections	36842
<I/σ(I)>	24.3	9
Completeness [%]	99.8	100
Redundancy	7	0.249

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	16% PEG 8000, 0.1M MGCL2, 0.1M HEPES PH 7.5