5J2L
De novo design of protein homo-oligomers with modular hydrogen bond network-mediated specificity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.380, 45.700, 48.320 |
| Unit cell angles | 90.00, 97.43, 90.00 |
Refinement procedure
| Resolution | 35.080 - 1.960 |
| R-factor | 0.2094 |
| Rwork | 0.206 |
| R-free | 0.24190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.126 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_1616) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.080 |
| High resolution limit [Å] | 1.960 |
| Rmerge | 0.056 |
| Number of reflections | 11050 |
| <I/σ(I)> | 23.1 |
| Completeness [%] | 99.4 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Tris pH 8.5 and 2.0 M Ammonium sulfate |
