5J1N

Lassa virus L protein cap-snatching endonuclease. Bound to one manganese ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.976
Spacegroup name	P 41 21 2
Unit cell lengths	51.500, 51.500, 143.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.450 - 1.090
R-factor	0.16178
Rwork	0.161
R-free	0.17931
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5izh
RMSD bond length	0.010
RMSD bond angle	1.498
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.450	1.120
High resolution limit [Å]	1.090	1.090
Rmerge	0.061	0.705
Number of reflections	80592
<I/σ(I)>	16.93
Completeness [%]	99.2
Redundancy	6.72

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	294	0.1 MES pH6 20% 2 Methyl-2,4,pentanediol 2mM MnCl2 10 mM GMP