5J1I

Structure of the spectrin repeats 7, 8, and 9 of the plakin domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-12-10
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9330
Spacegroup name	C 1 2 1
Unit cell lengths	75.240, 90.700, 154.540
Unit cell angles	90.00, 99.13, 90.00

Refinement procedure

Resolution	48.878 - 2.801
R-factor	0.2971
Rwork	0.296
R-free	0.32150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5j1g
RMSD bond length	0.005
RMSD bond angle	0.761
Data reduction software	XDS (November 3, 2014)
Data scaling software	XSCALE (November 3, 2014)
Phasing software	PHASER (2.3.0)
Refinement software	PHENIX (dev_2299)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.878	2.870
High resolution limit [Å]	2.800	2.800
Rpim	0.083	1.380
Number of reflections	14129
<I/σ(I)>	17.2	1.7
Completeness [%]	55.7	11.4
Redundancy	7.5	6.2
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	Protein solution at 12 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1 mM DTT was mixed with crystallization solution 0.1 M Bis-Tris-propane (pH 8.0), 16% PEG 3350, 0.3 M Na/K tartrate