5J1G

Structure of the spectrin repeats 7 and 8 of the plakin domain of plectin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-04-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97915
Spacegroup name	P 1 21 1
Unit cell lengths	45.680, 115.940, 64.800
Unit cell angles	90.00, 97.56, 90.00

Refinement procedure

Resolution	45.283 - 1.800
R-factor	0.1888
Rwork	0.187
R-free	0.21850
Structure solution method	SIRAS
RMSD bond length	0.008
RMSD bond angle	0.938
Data reduction software	XDS (March 1, 2015)
Data scaling software	XSCALE (March 1, 2015)
Phasing software	SHELXDE (2006/3)
Refinement software	PHENIX ((dev_2016: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	1.850
High resolution limit [Å]	1.800	1.800
Rpim	0.076	1.650
Number of reflections	61443
<I/σ(I)>	12	2
Completeness [%]	99.4	98.3
Redundancy	6.6	6.5
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.7	298	A protein solution at 27 mg/ml in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl was mixed with an equal volume of crystallization solution 50 mM Tris-HCl (pH 7.7), 0.2 M NaF, 18% PEG 3350