5J1A
Antigen presenting molecule
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-10-23 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.95370 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.186, 90.321, 106.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.470 - 1.860 |
R-factor | 0.1877 |
Rwork | 0.186 |
R-free | 0.21670 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.000 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER (4X6F) |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.470 | 1.900 |
High resolution limit [Å] | 1.860 | 1.860 |
Rmerge | 1.300 | |
Number of reflections | 34922 | |
<I/σ(I)> | 3.7 | |
Completeness [%] | 100 | |
Redundancy | 14.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20-25% PEG 1500 / 10% MMT buffer pH 5-6 |