5J0E
Crystal structures of Pribnow box consensus promoter sequence (P32)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-01 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5417 |
| Spacegroup name | P 32 |
| Unit cell lengths | 46.906, 46.906, 99.044 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.620 - 2.810 |
| R-factor | 0.22707 |
| Rwork | 0.226 |
| R-free | 0.24801 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 463d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.787 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.620 | 2.910 |
| High resolution limit [Å] | 2.810 | 2.810 |
| Rmerge | 0.028 | 0.136 |
| Number of reflections | 5955 | |
| <I/σ(I)> | 17.08 | 3.87 |
| Completeness [%] | 99.3 | 96.5 |
| Redundancy | 1.98 | 1.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MPD, sodium cacodylate, zinc chloride, spermine |
