5J08
Crystal structure of yeast Ent5 N-terminal domain-native P21
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.345, 51.758, 59.452 |
| Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
| Resolution | 28.960 - 1.800 |
| R-factor | 0.20086 |
| Rwork | 0.200 |
| R-free | 0.22002 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cmw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.251 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.306 |
| Number of reflections | 13631 | |
| <I/σ(I)> | 35.5 | 12.8 |
| Completeness [%] | 84.1 | 96.9 |
| Redundancy | 3.6 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 0.5M Potassium thiocyanate,0.1M BIS-TRIS propane pH 7.0 |
