5J07
Monomeric Human Cu,Zn Superoxide dismutase, loops IV and VII deleted, apo form, circular permutant P1/2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 75.040, 75.040, 59.010 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.510 - 2.000 |
| R-factor | 0.1946 |
| Rwork | 0.193 |
| R-free | 0.23060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bcz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.880 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | 0.618 |
| Number of reflections | 12893 | |
| <I/σ(I)> | 15.2 | |
| Completeness [%] | 100.0 | |
| Redundancy | 12.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 5 | 293 | 0.1 M MIB buffer pH 5.0 25 % w/v PEG 1500 |
