5IZV

Crystal structure of the legionella pneumophila effector protein RavZ - F222

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	293
Detector technology	CCD
Collection date	2014-01-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9794
Spacegroup name	F 2 2 2
Unit cell lengths	72.251, 313.003, 314.465
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.550 - 2.814
R-factor	0.1885
Rwork	0.188
R-free	0.22710
RMSD bond length	0.011
RMSD bond angle	1.486
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.550
High resolution limit [Å]	2.814
Number of reflections	42566
<I/σ(I)>	36.92
Completeness [%]	98.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	2M NaCl, 20% PEG 3350, 0.1M MgCl2, 0.1M Imidazole pH 6.5