5IZR
Human GIVD cytosolic phospholipase A2 in complex with Methyl gamma-Linolenyl Fluorophosphonate inhibitor and Terbium Chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.650 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.824, 112.667, 159.200 |
| Unit cell angles | 90.00, 90.03, 90.00 |
Refinement procedure
| Resolution | 159.200 - 3.250 |
| R-factor | 0.23174 |
| Rwork | 0.230 |
| R-free | 0.26654 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.291 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 159.200 | 3.340 |
| High resolution limit [Å] | 3.250 | 3.250 |
| Rmerge | 0.162 | |
| Number of reflections | 56076 | |
| <I/σ(I)> | 9.9 | 2.1 |
| Completeness [%] | 99.6 | 99.2 |
| Redundancy | 7.5 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 100 mM MES (pH 6.0-6.2), 10-14% (v/v) PEG20K and 50 mM sodium acetate |
