5IZN
The crystal structure of 50S ribosomal protein L25 from Vibrio vulnificus CMCP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.451, 58.797, 122.004 |
| Unit cell angles | 90.00, 90.51, 90.00 |
Refinement procedure
| Resolution | 34.220 - 2.350 |
| R-factor | 0.2072 |
| Rwork | 0.205 |
| R-free | 0.25210 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.447 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.089 | 0.843 |
| Number of reflections | 38178 | |
| <I/σ(I)> | 22.446 | 1.3 |
| Completeness [%] | 98.6 | 95.5 |
| Redundancy | 3.5 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 289 | 1.0M NaH2PO4/K2HPO4 |
