5IZM

The crystal structure of human eEFSec in complex with GDPNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	C 2 2 21
Unit cell lengths	92.316, 112.398, 327.669
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.158 - 3.400
R-factor	0.2379
Rwork	0.235
R-free	0.28480
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.755
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MLPHARE
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.158	3.460
High resolution limit [Å]	3.380	3.380
Number of reflections	24257
<I/σ(I)>	19.7
Completeness [%]	98.5
Redundancy	9.5
CC(1/2)	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		285	0.1 M HEPES pH 7.6, 0.3 M ammonium sulfate, 16% (w/v) PEG 3,350, 0.02 M glycine, 4% dextran sulfate