5IZE
Hantaan virus L protein cap-snatching endonuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | OTHER |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.984 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.200, 76.150, 63.720 |
| Unit cell angles | 90.00, 103.97, 90.00 |
Refinement procedure
| Resolution | 48.000 - 1.700 |
| R-factor | 0.16821 |
| Rwork | 0.166 |
| R-free | 0.21595 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.365 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.067 | 1.030 |
| Number of reflections | 41154 | |
| <I/σ(I)> | 11.99 | |
| Completeness [%] | 97.8 | |
| Redundancy | 3.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 316 | Hepes 0.1 M pH7, 20% PEG 6K, 1 M LiCl2 |
