5IZ5

Human GIVD cytosolic phospholipase A2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.987
Spacegroup name	P 62
Unit cell lengths	180.783, 180.783, 133.539
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	156.560 - 2.200
R-factor	0.22394
Rwork	0.223
R-free	0.23781
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.221
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	156.560	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.077
Number of reflections	125039
<I/σ(I)>	21.9	1.9
Completeness [%]	100.0	100
Redundancy	7.3	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		290	100 mM Bis-Tris-propane (pH 7.0-7.5), 1.4-1.5 M Lithium Sulphate