5IZ3

P. patens sedoheptulose-1,7-bisphosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-03-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0001
Spacegroup name	P 2 2 21
Unit cell lengths	45.180, 70.417, 197.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	98.620 - 1.300
R-factor	0.1612
Rwork	0.160
R-free	0.19080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d9q
RMSD bond length	0.025
RMSD bond angle	2.317
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	1.300
Rmerge	0.067
Number of reflections	155382
<I/σ(I)>	30.6
Completeness [%]	99.3
Redundancy	10.7
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	281	40 % PEG 600 0.2 M imidazole/malate