5IZ2
Crystal structure of the N. clavipes spidroin NTD at pH 6.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-17 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.480, 67.480, 90.350 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.741 - 2.020 |
| R-factor | 0.1685 |
| Rwork | 0.164 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3l2r |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.724 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.081 | 0.600 |
| Number of reflections | 16120 | |
| <I/σ(I)> | 21.71 | 5.57 |
| Completeness [%] | 98.2 | 94.32 |
| Redundancy | 10.81 | 11.06 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.1 M BIS-TRIS, 29% w/v Polyethylene glycol 3,350 |
