5IYY
X-ray structure of neuropilin-1 b1 domain complexed with Arg-4 ligand.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-02-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.525, 88.945, 41.700 |
| Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
| Resolution | 44.470 - 1.600 |
| R-factor | 0.14793 |
| Rwork | 0.146 |
| R-free | 0.18705 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kex |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.117 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.500 | 1.420 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rmerge | 0.137 | 0.854 |
| Number of reflections | 60739 | |
| <I/σ(I)> | 7.4 | 1.4 |
| Completeness [%] | 98.0 | 82.5 |
| Redundancy | 6.5 | 4.7 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 293 | 30% w/v PEG3350, 0.2 M AmCl |
