5IYV
Crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with the peptide hormone IDL1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-14 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999980 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 150.181, 150.181, 60.070 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.160 - 2.560 |
| R-factor | 0.19294 |
| Rwork | 0.191 |
| R-free | 0.23107 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.479 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.720 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 25124 | |
| <I/σ(I)> | 25.97 | 1.91 |
| Completeness [%] | 99.5 | 98.8 |
| Redundancy | 15 | 14.6 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 22% PEG 3350, 0.2 M MgCl2, 0.1 M citric acid pH 4.0 |
