5IYU
AlgE_CIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-23 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9993 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.830, 73.390, 114.530 |
| Unit cell angles | 90.00, 101.93, 90.00 |
Refinement procedure
| Resolution | 44.320 - 2.700 |
| R-factor | 0.2261 |
| Rwork | 0.223 |
| R-free | 0.28250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4afk |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.584 |
| Data reduction software | xia2 |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2283: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.320 | |
| High resolution limit [Å] | 2.700 | |
| Rmerge | 0.190 | 0.598 |
| Number of reflections | 12694 | |
| <I/σ(I)> | 4.7 | |
| Completeness [%] | 99.2 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293.15 | 38 %(v/v) PEG 400, 0.1 M NaCitrate pH 5.6 and 0.1 M bis-tris pH 5.0 |
