5IY4
Crystal structure of human PCNA in complex with the PIP box of DVC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9776 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 71.647, 71.647, 322.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.329 - 2.945 |
| R-factor | 0.2103 |
| Rwork | 0.207 |
| R-free | 0.27030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u7b |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.463 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.330 | 3.000 |
| High resolution limit [Å] | 2.945 | 2.950 |
| Rmerge | 0.160 | 0.770 |
| Number of reflections | 18902 | |
| <I/σ(I)> | 10.34 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.4 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 289 | 0.1 M Sodium citrate tribasic dehydrates (pH 5.0), 30% v/v Jeffamine ED-2001 (pH 7.0) |
