5IY2
Structure of apo OXA-143 carbapenemase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.955, 62.333, 87.093 |
| Unit cell angles | 90.00, 91.72, 90.00 |
Refinement procedure
| Resolution | 36.625 - 1.150 |
| R-factor | 0.1374 |
| Rwork | 0.136 |
| R-free | 0.16060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.820 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.625 | 1.180 |
| High resolution limit [Å] | 1.150 | 1.150 |
| Rmerge | 0.058 | 0.623 |
| Number of reflections | 150305 | |
| <I/σ(I)> | 11.45 | 1.9 |
| Completeness [%] | 96.1 | 87.8 |
| Redundancy | 3.4 | 3 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, 30% PEG 4000 |
