5IY0
PfMCM N-terminal domain double hexamer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 211.427, 122.610, 111.892 |
| Unit cell angles | 90.00, 92.35, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.20541 |
| Rwork | 0.205 |
| R-free | 0.22273 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pof |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.342 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.143 | 0.473 |
| Number of reflections | 57090 | |
| <I/σ(I)> | 6.4 | 2.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 295 | 0.1 M HEPES, pH 7.1; 0.1 Magnesium Chloride; 10% PEG 4000 |
