5IXZ

Crystal structure of the signaling protein complex 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-17
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 21 3
Unit cell lengths	123.267, 123.267, 123.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.980 - 2.400
R-factor	0.15038
Rwork	0.149
R-free	0.17747
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iam
RMSD bond length	0.007
RMSD bond angle	1.314
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.980	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.150
Number of reflections	23398
<I/σ(I)>	11.5	2.54
Completeness [%]	99.8	100
Redundancy	7.2	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	1.9M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K