5IXX

Crystal structure of the signaling protein complex 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-01
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.97945
Spacegroup name	P 21 3
Unit cell lengths	122.349, 122.349, 122.349
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.690 - 2.350
R-factor	0.11896
Rwork	0.116
R-free	0.16906
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4iam
RMSD bond length	0.011
RMSD bond angle	2.925
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.690	2.430
High resolution limit [Å]	2.350	2.350
Rmerge	0.160	0.230
Number of reflections	24332
<I/σ(I)>	12.2	9.7
Completeness [%]	99.8	100
Redundancy	7.3	7.4
CC(1/2)	0.955

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	295	1.9M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K