5IXW
Crystal structure of the signaling protein complex 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 122.830, 122.830, 122.830 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.070 - 2.460 |
| R-factor | 0.15589 |
| Rwork | 0.153 |
| R-free | 0.21122 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iam |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.598 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.070 | 2.550 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.134 | 0.550 |
| Number of reflections | 21331 | |
| <I/σ(I)> | 15 | 2.05 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 11.6 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 295 | 1.9M sodium malonate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
