5IXU

Crystal structure of an uncharacterized NIPSNAP domain protein from Burkholderia xenovorans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-16
Detector	RAYONIX MX-300
Wavelength(s)	0.97857
Spacegroup name	P 4 3 2
Unit cell lengths	94.620, 94.620, 94.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.199
Rwork	0.192
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vqs
RMSD bond length	0.008
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.570
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.063	0.023	0.569
Number of reflections	5419
<I/σ(I)>	43.75	66.23	6.65
Completeness [%]	99.7	95.6	100
Redundancy	26.79
CC(1/2)	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	290	JCSG+ E4: 1.26 M Ammonium sulfate, 0.1 M Tris pH 8.5; protein conc. 19.1mg/mL; 20% EG cryo; puck bbt8-3