5IXQ
Crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain in complex with the peptide hormone IDA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000010 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 148.282, 148.282, 57.930 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 128.420 - 1.860 |
| R-factor | 0.18415 |
| Rwork | 0.183 |
| R-free | 0.20729 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5ixo |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.420 | 1.970 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 61813 | |
| <I/σ(I)> | 16.7 | 2 |
| Completeness [%] | 99.8 | 98.6 |
| Redundancy | 20.3 | 19.1 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 19% PEG 3350, 0.2 M MgCl2, 0.1 M citric acid pH 4.0 |
