5IXP
Crystal structure of Extracellular solute-binding protein family 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.967, 115.184, 59.976 |
| Unit cell angles | 90.00, 131.17, 90.00 |
Refinement procedure
| Resolution | 33.862 - 1.730 |
| R-factor | 0.1324 |
| Rwork | 0.130 |
| R-free | 0.17150 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.815 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.730 | 4.690 | 1.730 |
| Rmerge | 0.044 | 0.034 | 0.562 |
| Number of reflections | 47739 | ||
| <I/σ(I)> | 13.9 | ||
| Completeness [%] | 99.7 | 98.7 | 97.9 |
| Redundancy | 4.4 | 4.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | Bis-Tris propane, tacsimate |
