5IXP

Crystal structure of Extracellular solute-binding protein family 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2012-11-07
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.97931
Spacegroup name	C 1 2 1
Unit cell lengths	89.967, 115.184, 59.976
Unit cell angles	90.00, 131.17, 90.00

Refinement procedure

Resolution	33.862 - 1.730
R-factor	0.1324
Rwork	0.130
R-free	0.17150
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	0.815
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.760
High resolution limit [Å]	1.730	4.690	1.730
Rmerge	0.044	0.034	0.562
Number of reflections	47739
<I/σ(I)>	13.9
Completeness [%]	99.7	98.7	97.9
Redundancy	4.4	4.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	Bis-Tris propane, tacsimate