5IXO
Crystal structure of the Arabidopsis receptor kinase HAESA LRR ectdomain (apo form).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000010 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 148.678, 148.678, 58.041 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 128.760 - 1.740 |
| R-factor | 0.18227 |
| Rwork | 0.180 |
| R-free | 0.22172 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.259 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 128.760 | 1.850 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 74928 | |
| <I/σ(I)> | 18.7 | 1.8 |
| Completeness [%] | 99.6 | 97.4 |
| Redundancy | 14.4 | 14 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 298 | 22% PEG 3350, 0.2 MgCl2, 0.1 M citric acid pH 4.0 |
