5IXH
Crystal Structure of Burkholderia cenocepacia BcnA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-15 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 61 |
| Unit cell lengths | 93.970, 93.970, 76.660 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.676 - 1.400 |
| R-factor | 0.1577 |
| Rwork | 0.156 |
| R-free | 0.18320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fgs |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.464 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.1.26) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.940 | 35.940 | 1.430 |
| High resolution limit [Å] | 1.400 | 7.410 | 1.400 |
| Rmerge | 0.063 | 0.041 | 0.545 |
| Rmeas | 0.066 | ||
| Rpim | 0.020 | ||
| Total number of observations | 796780 | ||
| Number of reflections | 75559 | ||
| <I/σ(I)> | 17.1 | ||
| Completeness [%] | 99.9 | 93.2 | 99.4 |
| Redundancy | 10.5 | 10.1 | 5.7 |
| CC(1/2) | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | Tris, ammonium sulphate |
