5IWQ

Crystal structure of aspartate aminotransferase (AspAT) from Corynebacterium glutamicum ATCC 13032

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-23
Detector	ADSC
Wavelength(s)	0.97934
Spacegroup name	C 1 2 1
Unit cell lengths	103.388, 53.521, 170.224
Unit cell angles	90.00, 104.23, 90.00

Refinement procedure

Resolution	165.000 - 2.000
R-factor	0.1509
Rwork	0.148
R-free	0.19860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d6k
RMSD bond length	0.019
RMSD bond angle	1.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	HKL-2000
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	165.000	2.030
High resolution limit [Å]	1.999	2.000
Number of reflections	56893
<I/σ(I)>	41.61
Completeness [%]	97.7
Redundancy	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350, Ammonium citrate