5IWP
Structure of Transient Receptor Potential (TRP) channel TRPV6 in the presence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-10-24 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.75 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 144.350, 144.350, 113.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.556 - 3.650 |
R-factor | 0.2731 |
Rwork | 0.273 |
R-free | 0.28320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2rfa |
RMSD bond length | 0.004 |
RMSD bond angle | 1.035 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.560 | 3.780 |
High resolution limit [Å] | 3.650 | 3.650 |
Rmerge | 0.010 | 1.431 |
Number of reflections | 25439 | |
<I/σ(I)> | 25 | 2.4 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 26.5 | 17.2 |
CC(1/2) | 0.995 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 20-24% PEG 350 MME, 50 mM NaCl, and 50 mM Tris-HCl pH 8.0-8.5 |