Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.920 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 151.720, 159.950, 168.570 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.330 |
| R-factor | 0.212 |
| Rwork | 0.209 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.490 | 3.450 |
| High resolution limit [Å] | 3.330 | 3.330 |
| Number of reflections | 29811 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.6 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 277 | 28% PEG300, 100 mM sodium acetate, pH 4.9 |
