Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-12-06 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.920 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 157.400, 160.870, 167.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.750 |
| R-factor | 0.231 |
| Rwork | 0.228 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4lto |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | |
| High resolution limit [Å] | 3.750 | 3.750 |
| Number of reflections | 21081 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.25 | 277 | 100 mM sodium acetate, pH 5.25, 30% PEG300 |
