5IWK

Structure of Transient Receptor Potential (TRP) channel TRPV6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.979
Spacegroup name	P 4 21 2
Unit cell lengths	143.818, 143.818, 113.224
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.464 - 3.247
R-factor	0.2625
Rwork	0.261
R-free	0.28830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2rfa
RMSD bond length	0.007
RMSD bond angle	1.208
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.464	3.360
High resolution limit [Å]	3.247	3.247
Rmerge	0.098	1.326
Number of reflections	18531
<I/σ(I)>	16.9	1.3
Completeness [%]	96.0	94.7
Redundancy	8.7	9.2
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	20-24% PEG 350MME, 50 mM NaCl, 50 mM Tris-HCl pH 8.0-8.5