5IW9

Structure of bacteriophage T4 gp25, sheath polymerization initiator

Replaces:  4HRZ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-09-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.97930
Spacegroup name	P 41 2 2
Unit cell lengths	69.859, 69.859, 222.179
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	69.860 - 2.470
R-factor	0.1911
Rwork	0.190
R-free	0.21720
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.506
Data reduction software	iMOSFLM (7.2.1)
Data scaling software	SCALA (0.5.23)
Phasing software	SHELXCD (2006/3)
Refinement software	PHENIX ((dev_2328: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.060	74.060	2.570
High resolution limit [Å]	2.470	8.910	2.470
Rmerge	0.085	0.043	0.843
Number of reflections	20716
<I/σ(I)>	30.8		5.2
Completeness [%]	100.0	99.5	100
Redundancy	23.4	19.1	24.8
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	291	0.9M-1.0M MG2SO4, 0.1M MES pH 6.2, protein concentration = 20 mg/ml